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Item #:
145280900
Bis-Tris Propane/HCl Buffer, pH 7.5 [1M] - 250mL
Item #:
145280900
PHP 24882
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What Stands Out
Optimal pH Stability
Designed to maintain a consistent pH of 7.5, ensuring reliable performance for biochemical experiments and applications requiring precision in pH control.
High Concentration
Contains 1M concentration for effective buffering capacity, making it ideal for various laboratory applications including electrophoresis and protein studies.
Convenient Size
Packaged in a 250mL bottle for easy handling and storage, providing ample solution for repeated use in experimental setups without frequent repurchasing.
Product Details
| Item Weight | 0.55 lbs (250 grams) |
Who Should Buy?
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Biochemical Research
Ideal for researchers conducting experiments requiring a stable pH near 7.5 for biochemical analyses.
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Protein Studies
Suitable for studies involving proteins, as it maintains a favorable environment for protein stability.
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Molecular Biology
Useful in molecular biology protocols that require reliable buffering at a neutral pH.
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Extreme pH Environments
Not suitable for applications needing a pH outside the 6-8 range as stability may be compromised.
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Basic Chemistry Labs
Less effective for non-biological applications or basic chemistry experiments unrelated to biological systems.
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Long-term Storage
Not recommended for long-term storage of sensitive biomolecules due to potential pH shifts over time.
Product Description
Bis-Tris Propane/HCl Buffer, pH 7.5 [1M] - 250mL
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Customer Questions & Answers
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Question:
What is Bis-Tris Propane/HCl Buffer used for?
Answer: Bis-Tris Propane/HCl Buffer is primarily used for biochemical and molecular biology applications, particularly in protein purification and analysis. Its pH stability allows researchers to maintain consistent conditions for protein functions and enzymatic reactions. In practice, this buffer is ideal for preparing protein samples for electrophoresis or chromatography, ensuring accurate and reproducible results in experimental protocols. -
Question:
How should Bis-Tris Propane/HCl Buffer be stored?
Answer: Bis-Tris Propane/HCl Buffer should be stored at room temperature, away from direct sunlight and moisture to maintain its stability. Proper storage conditions ensure that the buffer maintains its intended pH and concentration. For optimal longevity, keep it in a tightly sealed container. This is crucial in laboratory settings, where maintaining buffer integrity can significantly affect experimental outcomes. -
Question:
Can Bis-Tris Propane/HCl Buffer be used with different biological samples?
Answer: Yes, this buffer is versatile and suitable for use with a variety of biological samples including proteins, nucleic acids, and enzymes. Its compatibility allows researchers to use it effectively in diverse applications such as Western blots, electrophoresis, and other analytical techniques. This adaptability makes it a preferred choice in laboratories working with different biological materials. -
Question:
What are the benefits of using Bis-Tris Propane/HCl over other buffers?
Answer: Bis-Tris Propane/HCl offers several advantages, including excellent buffering capacity at a physiological pH range and minimal interference with biological processes. Unlike some other buffers, it does not chelate metal ions, making it particularly suitable for protein studies where metals may be involved. Researchers often prefer it for its reliability in maintaining stable pH during experiments, which is critical for sensitive biological assays. -
Question:
Is Bis-Tris Propane/HCl Buffer compatible with all protein types?
Answer: In general, Bis-Tris Propane/HCl Buffer is compatible with a wide range of proteins, particularly those sensitive to pH variations. It provides an environment that stabilizes proteins and is preferred for many purification and analytical techniques. However, it is always recommended to validate compatibility for specific proteins and experimental setups to ensure optimal results. -
Question:
What is the ideal concentration for using Bis-Tris Propane/HCl Buffer?
Answer: Bis-Tris Propane/HCl Buffer is typically used at a 1M concentration for most applications. This concentration provides effective buffering capacity, maintaining pH stability under varying conditions. In practical scenarios, researchers might dilute this buffer to lower concentrations depending on specific experimental requirements, while still enjoying its robust buffering characteristics. -
Question:
Can I use Bis-Tris Propane/HCl Buffer in sensitive enzymatic reactions?
Answer: Yes, Bis-Tris Propane/HCl Buffer is particularly suitable for sensitive enzymatic reactions due to its minimal inhibitory effects on enzyme activity and effective pH stabilization. This makes it an excellent choice for reactions involving delicate enzymes, as it helps maintain optimal conditions for activity, ensuring reliable and reproducible experimental results. -
Question:
What pH adjustments can be made with Bis-Tris Propane/HCl Buffer?
Answer: Bis-Tris Propane/HCl Buffer can be adjusted to stable pH levels typically between 6.5 and 8.5, making it versatile for various applications. Adjustments can be made using hydrochloric acid or sodium hydroxide. This flexibility allows researchers to tailor the buffer to meet specific experimental conditions, accommodating the unique pH requirements of different biological systems. -
Question:
Who should use Bis-Tris Propane/HCl Buffer?
Answer: Bis-Tris Propane/HCl Buffer is ideal for researchers, students, and professionals in molecular biology, biochemistry, and related fields. Its reliability and effectiveness in maintaining pH make it a vital tool in experimental design. Whether you’re working on protein purification, enzyme assays, or academic research, this buffer can support accurate scientific investigations. -
Question:
Where can I buy Bis-Tris Propane/HCl Buffer, pH 7.5 [1M] - 250mL?
Answer: You can purchase Bis-Tris Propane/HCl Buffer, pH 7.5 [1M] - 250mL from Ubuy in Philippines. Ubuy provides a reliable platform with extensive product offerings, making it easy for you to find and order the specific chemicals and lab supplies you need for your research or laboratory work.
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PHP 24882
Order now and get it around Monday, July 06
This item is not restrict in my country.(Please click on above link if this item is not restrict in your country, So our team will review and allow.)
QTY:
Secured transaction
Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.
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Features & Benefits
- Wide buffering range (pH 6.3-9.5) for versatile applications.
- Manufactured in an ISO 9001 and 13485 certified facility in the USA.
- Strict adherence to Good Manufacturing Practices.
- Quality control tests conducted under certified clean room conditions.
- Ideal for protein research, molecular biology, and characterization studies.
- Not for human or animal clinical/therapeutic use.