What is EGTA and its primary use?

EGTA, or Ethylene Glycol-bis(2-aminoethylether)-N,N,N',N-tetraacetic Acid, is a chelating agent used primarily to bind calcium ions in biological and chemical applications. Its unique structure allows it to effectively sequester calcium, making it invaluable in molecular biology, biochemistry, and biophysics for experiments. For instance, EGTA is often utilized in cell culture and enzyme activity assays where calcium's presence could interfere with results.

How does EGTA differ from EDTA?

EGTA is similar to EDTA but specifically designed to bind calcium ions more effectively while having a much lower affinity for magnesium and other metal ions. This property makes EGTA particularly suitable for experiments where you want to selectively remove calcium, allowing for more controlled studies. Researchers favor EGTA in protocols that require tight regulation of calcium levels, such as studying calcium-dependent enzymes.

Can EGTA be used in educational labs?

Yes, EGTA is frequently used in educational laboratories for teaching purposes, particularly in courses involving biochemistry and molecular biology. As it can help demonstrate how chelating agents work, students can engage in hands-on experiments observing the effects of calcium removal. These practical applications foster a better understanding of complex biochemical processes.

Is EGTA suitable for industrial applications?

Absolutely, EGTA is employed in various industrial processes, particularly in water treatment and analytical chemistry. Its capacity to sequester calcium helps in controlling hardness in water, which can prevent scaling in pipes and machinery. Additionally, EGTA finds usage in formulating cleaning agents where calcium removal is necessary to enhance effectiveness.

What safety precautions should be taken when handling EGTA?

While EGTA is generally recognized as safe for laboratory use, it is essential to handle it with appropriate precautions. Always wear gloves, goggles, and lab coats to protect against skin or eye irritation. It is advisable to work in a well-ventilated area to minimize inhalation exposure. Familiarize yourself with the material safety data sheet (MSDS) for detailed handling and first aid measures.

How should EGTA be stored?

EGTA should be stored in a cool, dry place away from direct sunlight and moisture, ideally in its original container or a tightly sealed glass or plastic bottle. Proper storage conditions help maintain its stability and effectiveness over time. Make sure to label containers clearly to prevent accidental misuse or contamination.

Can EGTA be used in combination with other reagents?

EGTA can be used alongside various reagents in experimental setups, but it’s crucial to consider the interactions with other chemicals. For example, in biochemical assays, adding EGTA can help inhibit calcium-dependent processes while allowing other reactions to proceed unhindered. Researchers should always conduct preliminary tests to verify compatibility in their specific applications.

What are the common applications of EGTA in research?

EGTA has widespread applications in research, including enzyme assays, protein purification, and studying calcium-mediated signaling pathways. Its ability to precisely bind calcium allows researchers to dissect cellular mechanisms and observe resultant changes in activity or function that are influenced by calcium levels. These studies provide insight into critical biological processes and disease mechanisms.

Is EGTA solubility a concern?

EGTA is soluble in water, but its solubility can be influenced by pH and temperature. When preparing solutions, it is often best to dissolve it in a buffer solution that mimics physiological conditions to ensure full solubility and biological relevance. Researchers need to account for these factors during preparation to ensure accurate experimental conditions.

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RPI

EGTA [Ethylene Glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic Acid], 100 Grams

Product is sold for research or further manufacturing use only.

RPI

Item #: 172097469

EGTA [Ethylene Glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic Acid], 100 Grams

Q: Where can I buy EGTA [Ethylene Glycol-bis(2-aminoethylether)-N,N,N',N-tetraacetic Acid], 100 Grams?

You can purchase EGTA [Ethylene Glycol-bis(2-aminoethylether)-N,N,N',N-tetraacetic Acid], 100 Grams on Ubuy. Ubuy is a reliable online platform that offers a wide range of chemical products, including research reagents. Their efficient service ensures that you can access this essential laboratory chemical conveniently within Philippines.

Item #: 172097469

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What Stands Out

High Purity

This EGTA boasts exceptional purity, ensuring reliable and consistent experiment results, making it ideal for sensitive biochemical applications.

Versatile Application

EGTA's unique chelation properties allow it to effectively bind calcium ions, making it invaluable for diverse applications in enzymology, molecular biology, and electrophysiology.

Convenient Packaging

Packaged in a 100-gram quantity, this EGTA offers convenience for laboratories, reducing handling and waste while maintaining effectiveness for various experiments.

Product Details

Brand Name	RPI
Manufacturer	RPI, Corp.
Part Number	E57060-100.0
Item Weight	2.24 ounces (63.5 grams)
Brand	RPI

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Who Should Buy?

Laboratory Researchers
Ideal for researchers needing a chelating agent for metal ion studies and biochemical experiments.
Analytical Chemists
Useful for chemists analyzing metal-content in samples, improving accuracy with its chelation properties.
Biochemists
Beneficial for biochemists performing assays that require metal ion modulation or stabilization in biological samples.

Non-Scientific Users
Not suitable for casual users lacking a background in chemistry or laboratory techniques.

Product Description

EGTA [Ethylene Glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic Acid], 100 Grams

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Question: How to Shop EGTA [Ethylene Online From Ubuy?

Answer: It’s easy to shop EGTA [Ethylene online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is EGTA [Ethylene Available to Shop Online in Philippines?

Answer: Yes, at Ubuy Philippines this product is available for you to shop at a reasonable price. The EGTA [Ethylene is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

RPI Acids Editorial Review

EGTA [Ethylene Glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic Acid], 100 Grams is a high-purity chemical compound produced by RPI, a reputable brand known for its quality products. This product weighs 2.24 ounces and is ideal for various laboratory applications including biochemistry and molecular biology. It's important to note that proper handling and usage guidelines should be followed when working with chemicals such as EGTA. Many users have expressed satisfaction with its reliability and effectiveness in their experiments, making it a trusted choice for researchers and scientists alike.

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Pros

High-purity chemical compound for laboratory use
Reputable brand known for quality products
Ideal for biochemical and molecular biology applications
Reliable and effective for various experiments
Convenient packaging for easy handling

Cons

Limited information on specific applications available

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Features & Benefits

CAS Number: 67-42-5 for easy identification.
Molecular Formula: C14H24N2O10 for precise chemical representation.
Molecular Weight: 380.35 for accurate measurements.
Ideal for research purposes and further manufacturing.
Not intended for food or drug use.
High-quality chemical for professional applications.

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EGTA [Ethylene Glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic Acid], 100 Grams

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